Watch Eminem's full cypher below.
The "Late Night" host said his viewers should "get off the fence" and pick a side between President Trump and his show, which he said "constantly mocks and denigrates everything about [Trump]".
The rapper took aim at Mr. Trump in a 4½-minute freestyle rap video that aired as part of BET's Hip Hop Awards on Tuesday night.
"Do you support him?" the NBC host continued.
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Eminem also targeted those who claimed to be supporters of both him and Trump, telling them they had to choose to either stand beside him or the president.
James Corden also imagined Trump's rap-response to Eminem's diss track, which borrowed from "The Real Slim Shady".
After playing the clip in which Trump called the rapper "a victor", Kimmel added, "Not anymore, I guess".
At the end of his rap, he insisted his fans should quit supporting the president.
What, you thought Eminem would let all the attention from a blistering, anti-Donald Trump freestyle go to waste? "Any fan of mine who's a supporter of his/ I'm drawing in the sand a line, you're either for or against/ And if you can't decide who you like more and you're split/ On who you should stand beside, I'll do it for you with this: F**k you, " read the lyrics.