The evacuation area included the Natural History Museum, the BND intelligence agency, a clinic of the Charite Hospital and an army hospital.
United Kingdom bomb, which is now being defused, was found at Heidestrasse street, after which police ordered all the buildings located within a 800-meter (over 2,600 feet) radius of the explosive to be evacuated, Der Tagesspiegel media outlet reported.
Police also went house to house to check the zone has been completely cleared before the bomb disposal experts began their work.
German police experts work during the disposal operations of a bomb dropped during World War II on April 20, 2018 near the Hauptbahnhof main railway station in Berlin's Mitte district.
A Russian-made aerial bomb weighing 100 kilograms was found near a train track leading into Berlin's main station in 2013.
Some 10,000 people have been evacuated from central Berlin so experts can defuse a huge World War Two bomb.
The operation is associated with defusing a bomb. "It depends on how long the evacuation takes and of course the condition of the bomb", police spokesman Martin Halweg told the Tagesspiegel daily. All streets in this area will be closed off. Travel disruption is expected around the area and at the central train station.
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Even 73 years after the end of the war, such discoveries remain common in major German cities.
Thousands of unexploded war time bombs still lie beneath cities in Germany.
Shelters have been set up for those affected.
A Deutsche Bahn spokesperson said they were preparing for interruptions to traffic.
Police said a number of WW2 bombs had found in this area earlier too.
Past year some 60,000 people were evacuated from their homes in Frankfurt after a massive bomb dropped by Britain's Royal Air Force was unearthed.